Journal: Journal of Biomedicine and Biotechnology

Article Title: Differential Proteome Analysis of the Preeclamptic Placenta Using Optimized Protein Extraction

doi: 10.1155/2010/458748

Figure Lengend Snippet: Two-dimensional separation of pooled proteins from normotensive (a) and PE (b). Circles indicate spots whose amount was increased in the 2D-PAGE of normotensive and PE samples analyzed with the Ludesi's software Redfin. Arrows show identified proteins; see . The gels were stained with SYPRO Ruby.

Article Snippet: The images of the large gels stained with SYPRO Ruby were submitted to Ludesi 2D Analysis (Ludesi, Malmo, Sweden, http://www.ludesi.com ) for spot detection, matching, and analysis.

Techniques: Software, Staining

Journal: Antibiotics

Article Title: Free-Floating Aggregate and Single-Cell-Initiated Biofilms of Staphylococcus aureus

doi: 10.3390/antibiotics10080889

Figure Lengend Snippet: Schematics of experimental setup for growing biofilm in a flow through system.

Article Snippet: For staining the biofilm components, wheat germ agglutinin alexa fluor 647 (W32466, Fisher Scientific, USA; final concentration, 5 μg/mL), DAPI solution (EN62248, Fisher Scientific, USA; final concentration, 1 μg/mL), and SYPRO ruby biofilm matrix stain (F10318, Fisher Scientific, USA) were used to stain the poly-N-acetylglucosamine (PNAG) or SF-derived hyaluronic acid (HA) residues, extracellular DNA (eDNA), and matrix proteins, respectively.

Techniques:

Journal: Antibiotics

Article Title: Free-Floating Aggregate and Single-Cell-Initiated Biofilms of Staphylococcus aureus

doi: 10.3390/antibiotics10080889

Figure Lengend Snippet: Confocal images of aggregated ( A , B ) and single cells ( C , D ) biofilms for 10 8 CFU/mL. Panel B and D are zoomed images. Biofilms stained with DAPI for eDNA, WGA stain for PNAG or SF-derived HA, and SYPRO for staining proteins contained in the biofilm matrix. Merged images denote the combination of GFP, DAPI, WGA, and SYPRO images.

Article Snippet: For staining the biofilm components, wheat germ agglutinin alexa fluor 647 (W32466, Fisher Scientific, USA; final concentration, 5 μg/mL), DAPI solution (EN62248, Fisher Scientific, USA; final concentration, 1 μg/mL), and SYPRO ruby biofilm matrix stain (F10318, Fisher Scientific, USA) were used to stain the poly-N-acetylglucosamine (PNAG) or SF-derived hyaluronic acid (HA) residues, extracellular DNA (eDNA), and matrix proteins, respectively.

Techniques: Staining, Derivative Assay

Journal: Antibiotics

Article Title: Free-Floating Aggregate and Single-Cell-Initiated Biofilms of Staphylococcus aureus

doi: 10.3390/antibiotics10080889

Figure Lengend Snippet: Confocal images of aggregated ( A , B ) and single cells ( C , D ) biofilms for 10 3 CFU/mL. Panel B and D are zoomed images. Biofilms stained with DAPI for eDNA, WGA stain for PNAG or SF-derived HA, and SYPRO for staining proteins contained in the biofilm matrix. Merged images denote the combination of GFP, DAPI, WGA, and SYPRO images.

Article Snippet: For staining the biofilm components, wheat germ agglutinin alexa fluor 647 (W32466, Fisher Scientific, USA; final concentration, 5 μg/mL), DAPI solution (EN62248, Fisher Scientific, USA; final concentration, 1 μg/mL), and SYPRO ruby biofilm matrix stain (F10318, Fisher Scientific, USA) were used to stain the poly-N-acetylglucosamine (PNAG) or SF-derived hyaluronic acid (HA) residues, extracellular DNA (eDNA), and matrix proteins, respectively.

Techniques: Staining, Derivative Assay

Journal: Antibiotics

Article Title: Free-Floating Aggregate and Single-Cell-Initiated Biofilms of Staphylococcus aureus

doi: 10.3390/antibiotics10080889

Figure Lengend Snippet: Confocal images of aggregated ( A , B ) and single cells ( C , D ) biofilms for 10 8 CFU/mL after 3 h antibiotic treatment. Panel B and D are zoomed images. Biofilms stained with DAPI for eDNA, WGA stain for PNAG or SF-derived HA, and PI for staining dead cells. Merged images denote the combination of DAPI, WGA, GFP, and PI images. GFP-PI is combination of GFP and PI images.

Article Snippet: For staining the biofilm components, wheat germ agglutinin alexa fluor 647 (W32466, Fisher Scientific, USA; final concentration, 5 μg/mL), DAPI solution (EN62248, Fisher Scientific, USA; final concentration, 1 μg/mL), and SYPRO ruby biofilm matrix stain (F10318, Fisher Scientific, USA) were used to stain the poly-N-acetylglucosamine (PNAG) or SF-derived hyaluronic acid (HA) residues, extracellular DNA (eDNA), and matrix proteins, respectively.

Techniques: Staining, Derivative Assay

Journal: Antibiotics

Article Title: Free-Floating Aggregate and Single-Cell-Initiated Biofilms of Staphylococcus aureus

doi: 10.3390/antibiotics10080889

Figure Lengend Snippet: Confocal images of aggregated ( A , B ) and single cells ( C , D ) biofilms for 10 8 CFU/mL after 24 h antibiotic treatment. Panel B and D are zoomed images. Biofilms stained with DAPI for eDNA, WGA stain for PNAG or SF-derived HA, and PI for staining dead cells. Merged images denote the combination of DAPI, WGA, GFP, and PI images. GFP-PI is combination of GFP and PI images.

Article Snippet: For staining the biofilm components, wheat germ agglutinin alexa fluor 647 (W32466, Fisher Scientific, USA; final concentration, 5 μg/mL), DAPI solution (EN62248, Fisher Scientific, USA; final concentration, 1 μg/mL), and SYPRO ruby biofilm matrix stain (F10318, Fisher Scientific, USA) were used to stain the poly-N-acetylglucosamine (PNAG) or SF-derived hyaluronic acid (HA) residues, extracellular DNA (eDNA), and matrix proteins, respectively.

Techniques: Staining, Derivative Assay

Journal: Antibiotics

Article Title: Free-Floating Aggregate and Single-Cell-Initiated Biofilms of Staphylococcus aureus

doi: 10.3390/antibiotics10080889

Figure Lengend Snippet: Confocal images of aggregated ( A , B ) and single cells ( C , D ) biofilms for 10 3 CFU/mL after 3 h antibiotic treatment. Panel B and D are zoomed images. Biofilms stained with DAPI for eDNA, WGA stain for PNAG or SF-derived HA, and PI for staining dead cells. Merged images denote the combination of DAPI, WGA, GFP, and PI images. GFP-PI is combination of GFP and PI images.

Article Snippet: For staining the biofilm components, wheat germ agglutinin alexa fluor 647 (W32466, Fisher Scientific, USA; final concentration, 5 μg/mL), DAPI solution (EN62248, Fisher Scientific, USA; final concentration, 1 μg/mL), and SYPRO ruby biofilm matrix stain (F10318, Fisher Scientific, USA) were used to stain the poly-N-acetylglucosamine (PNAG) or SF-derived hyaluronic acid (HA) residues, extracellular DNA (eDNA), and matrix proteins, respectively.

Techniques: Staining, Derivative Assay

Journal: Antibiotics

Article Title: Free-Floating Aggregate and Single-Cell-Initiated Biofilms of Staphylococcus aureus

doi: 10.3390/antibiotics10080889

Figure Lengend Snippet: Confocal images of aggregated ( A , B ) and single cells ( C , D ) biofilms for 10 3 CFU/mL after 24 h antibiotic treatment. Panel B and D are zoomed images. Biofilms stained with DAPI for eDNA, WGA stain for PNAG or SF-derived HA, and PI for staining dead cells. Merged images denote the combination of DAPI, WGA, GFP, and PI images. GFP-PI is combination of GFP and PI images.

Article Snippet: For staining the biofilm components, wheat germ agglutinin alexa fluor 647 (W32466, Fisher Scientific, USA; final concentration, 5 μg/mL), DAPI solution (EN62248, Fisher Scientific, USA; final concentration, 1 μg/mL), and SYPRO ruby biofilm matrix stain (F10318, Fisher Scientific, USA) were used to stain the poly-N-acetylglucosamine (PNAG) or SF-derived hyaluronic acid (HA) residues, extracellular DNA (eDNA), and matrix proteins, respectively.

Techniques: Staining, Derivative Assay